Medical grade collagen has a long history of use in surgical and implantable medical devices due to its high purity and preserved triple helix structure. TheraDep is now bringing this proven biomaterial into cell culture applications through its BioDep plasma coating process, creating medical grade collagen–coated microplates designed for improved in vitro performance.
Structural analysis confirms that medical grade collagen retains its native three-dimensional structure when deposited onto microplates, resulting in increased surface roughness compared to commercially available collagen coatings. This structural integrity supports enhanced cell attachment and more biologically relevant cell behavior.
Comparative studies using multiple assays, including MTS, resazurin, and crystal violet, show that cells grown on medical grade collagen demonstrate improved proliferation and greater consistency across wells. Additional testing with HUVEC, L929, and monocyte cell lines further confirms higher cell mass and more repeatable results.
Importantly, the combination of medical grade collagen and the BioDep coating process significantly reduces well-to-well variability, addressing a common challenge in cell culture experiments. This improved reproducibility supports more reliable data and easier translation from research to medical device development.
By leveraging a collagen source already used in millions of medical procedures, medical grade collagen–coated microplates provide researchers with a more consistent and clinically relevant surface for cell culture studies.
